Journal: Genomics Data

Article Title: Expression-profiling of apoptosis induced by ablation of the long ncRNA TRPM2-AS in prostate cancer cell

doi: 10.1016/j.gdata.2014.10.020

Figure Lengend Snippet: Validation of microarray data by qRT-PCR. Ten transcripts were quantified by qRT-PCR both in control and in TRPM2-AS KO PC3 cells. The resulting expression fold change is plotted against the expression fold change obtained from the Illumina HumanHT-12 V3.0 microarray data. A correlation coefficient of 0.97 was found between the two datasets.

Article Snippet: The human, androgen-independent prostate cancer cell line PC3 was obtained from ATCC (CRL-1435, ATCC).

Techniques: Biomarker Discovery, Microarray, Quantitative RT-PCR, Control, Expressing

Journal: Genomics Data

Article Title: Expression-profiling of apoptosis induced by ablation of the long ncRNA TRPM2-AS in prostate cancer cell

doi: 10.1016/j.gdata.2014.10.020

Figure Lengend Snippet:

Article Snippet: The human, androgen-independent prostate cancer cell line PC3 was obtained from ATCC (CRL-1435, ATCC).

Techniques: Expressing, Microarray, Gene Expression

Journal: Oncotarget

Article Title: Isolation and genome-wide expression and methylation characterization of CD31 + cells from normal and malignant human prostate tissue

doi:

Figure Lengend Snippet: (A) Representative photomicrographs of hematoxylin and eosin stained frozen sections of benign (left) and malignant (right) prostatic tissues of macrodissected tissues used for ECs isolation. ECs are highlighted in the frozen sections by CD31 immunostaining (Original magnification, x200). (B) Representative immunofluorescent photomicrographs of CD31 (green) and vWF (red) expression and uptake of DiI-Ac-LDL (red) (original magnification, ×200) in CD31 + prostate TdECs and NdECs. Absence of pan-cytokeratin expression by immunofluorescence was observed in CD31 + prostate TdECs and NdECs. LNCaP was used as positive control for pan-cytokeratin immunofluorescence. Nuclei are stained with DAPI (blue). Endothelial tube network was formed by primary cultures of prostate TdECs and NdECs (original magnification, x100). (C) Representative reverse transcription-PCR analysis of RNA from primary cultures of TdECs and NdECs, HUVECs, or LNCaP cells using human-specific primers for human CD31, CD34, ICAM-1, VCAM-1, VEGFR1, VEGFR2, AR and PSA. GAPDH was used as a loading control.

Article Snippet: Primary cultures of human prostate endothelial cells (NdECs and TdECs) were cultured in endothelial growth medium [Endothelial Cell Growth Medium MV2 with Supplement Mix (PromoCell GmbH, Heidelberg, Germany) and 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen)].

Techniques: Staining, Isolation, Immunostaining, Expressing, Immunofluorescence, Positive Control

Journal: Oncotarget

Article Title: Isolation and genome-wide expression and methylation characterization of CD31 + cells from normal and malignant human prostate tissue

doi:

Figure Lengend Snippet: Quantitative real-time PCR was used to validate gene expression of AREG , JMY , EPB41 , GMNN and FAM53C in endothelial cells derived from malignant lesions vs benign lesions in AA and CA patients with prostate cancer. Relative gene expression level for qRT-PCR was normalized to the reference gene GAPDH . Gene expression from microarray was plotted together with the qRT-PCR results. Results were shown as Mean ± SD (triplicate). “*” represents p value <0.05 by t-Test.

Article Snippet: Primary cultures of human prostate endothelial cells (NdECs and TdECs) were cultured in endothelial growth medium [Endothelial Cell Growth Medium MV2 with Supplement Mix (PromoCell GmbH, Heidelberg, Germany) and 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen)].

Techniques: Real-time Polymerase Chain Reaction, Expressing, Derivative Assay, Quantitative RT-PCR, Microarray

Journal: Carcinogenesis

Article Title: Muscadine grape skin extract can antagonize Snail-cathepsin L-mediated invasion, migration and osteoclastogenesis in prostate and breast cancer cells

doi: 10.1093/carcin/bgv084

Figure Lengend Snippet: CatL expression increases with tumor progression. (A) Immunohistochemical (IHC) analysis was performed using a 96 core prostate adenocarcinoma tissue microarray. Representive images of CatL in various stages of prostate cancer show that CatL increases with tumor progression. Bar represents 50 µM. (B) Normal/tumor matched invasive ductal carcinoma (IDC) grades 1 and 3, infiltrating carcinoma (IFC) grade 3, adenocarcinoma (grade 3) and metaplastic carcinoma (MPC) grade 3 were analyzed for expression of CatL by western blot analysis. Mature CatL expression was generally higher in tumor as compared to normal tissue. Alpha (α)-tubulin was used as a loading control. Data are representative of at least three independent experiments.

Article Snippet: Immunohistochemistry Examination of the expression and distribution of CatL in human prostate cancer was performed by immunohistochemistry (IHC) using prostate normal and cancer tissue microarray slides obtained from US Biomax (PR956a, Rockville, MD).

Techniques: Expressing, Immunohistochemical staining, Microarray, Western Blot